Analysis Note

ControlJurkat cells

Application

Research CategorySignaling

Research Sub CategoryMAP Kinases

Milli-Mark Anti-MAPK 1/2 (tERK) Antibody, Alexa Fluor 647 Conjugate detects level of MAPK 1/2 (tERK) & has been published & validated for use in FC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called MAPK1 and MAPK2, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or MAPK1/2. The MAPK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of MAPK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to rat MAPK 1/2 at the C-terminus.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit polyclonal antibody conjugated to Alexa Fluor 647 in PBS buffer with 15mg/mL BSA and 0.1% Sodium Azide

Quality

Evaluated by flow cytometry using Jurkat cells

Specificity

Antibody recognizes MAPK1/2. C-terminus.

Storage and Stability

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Target description

43 kDa Calculated